rabbit anti trpm5  (Proteintech)

Journal: Cell and tissue research

Article Title: Identification of cholinergic cells with chemosensory traits in the porcine uterus

doi: 10.1007/s00441-022-03585-1

Figure Lengend Snippet: Distribution and molecular signature of presumptive cholinergic chemosensory cells in the porcine uterus. Double-labeling for TRPM5 (red; a and d) and ChAT (green; b and e) revealed immunoreactive cells (arrows) localized to the endometrial luminal (a–c) and glandular (d–f) epithelia. Merged channels are shown (c and f). Note the enrichment of TRPM5 labeling in the apical, lumen-oriented pole of the cell (asterisks are positioned in the lumen). Inserts (a′–f′): the flask-shaped cell morphology of presumptive chemosensory cells at higher magnification. Nuclei are in blue. Scale bars equal 50 μm. Adjustments were made to black balance for image acquisition. Pre-absorption of TRPM5 antisera with its cognate peptide eliminated specific labeling (d″–f″). Western blot demonstrating TRPM5 in the porcine uterus (g). A single band of calculated size of 176.8 kDa was detected in uterus and trachea (positive control) homogenates. Beta-actin blotting was used as load control (g′)

Article Snippet: Uterine sections were immunolabeled with the following antibodies: mouse anti-ChAT (MAB5270, Millipore, Billerica, MA; 1:500 dilution), rabbit anti-beta-III tubulin (AB18207, Abcam, Cambridge, UK; 1:1500 dilution), rabbit anti-PGP9.5 (PA1–46205, Thermo Fisher; 1:1000 dilution), rabbit anti-TRPM5 (MBS3202490, MyBioSource, San Diego, CA; 1:500), rabbit anti-PLCB2 (MBS9606175, MyBioSource; 1:500), or mouse anti-acetylated alpha tubulin (MABT868; Millipore; 1:500 dilution).

Techniques: Labeling, Western Blot, Positive Control, Control